How Much Did BillionToOne Raise?
Funding & Key Investors

BillionToOne, a Menlo Park-based leader in precision diagnostics, has successfully navigated multiple capital raises to reach its current market position. The company has secured a cumulative $646.6M in financing, bolstered by a recent $273.1M strategic investment. This trajectory reflects strong investor confidence in the firm's proprietary molecular counting technology and its potential to redefine the landscape of noninvasive prenatal screening and broader molecular diagnostics.

What is BillionToOne?

BillionToOne
Business ServicesResearch & DevelopmentHealthcare Services

BillionToOne specializes in the development of its patent-pending QCT molecular counter platform, a unique technological advancement capable of counting DNA molecules at the single-count level. By enhancing the accuracy and accessibility of molecular diagnostics, the company has established a significant footprint in the healthcare sector. Its flagship product, UNITY, represents a breakthrough in prenatal care, enabling the detection of cystic fibrosis, spinal muscular atrophy, and hemoglobinopathies through maternal blood. This innovation positions the company as a critical player in the shift toward more efficient, noninvasive clinical diagnostics.

How much funding has BillionToOne raised?

BillionToOne has raised a total of $646.6M across 6 funding rounds:

2019

Series A

$15M

2021

Series B

$55M

2022

Series C

$125M

Unspecified

$48.5M

2024

Series D

$130M

2025

IPO

$273.1M

Series A (2019): $15M with participation from Hummingbird Ventures, Y Combinator, HOF Capital, 500 Startups Istanbul, NeoTribe Ventures, Libertus Capital, Civilization Ventures, and Pacific 8 Ventures

Series B (2021): $55M, investors not publicly disclosed

Series C (2022): $125M supported by Baillie Gifford, Neotribe Ventures, Adams Street Partners, and Hummingbird Ventures

Unspecified (2022): $48.5M featuring Libertus Capital, Hummingbird Ventures, Adams Street Partners, Baillie Gifford, and Neotribe Ventures

Series D (2024): $130M, investors not publicly disclosed

IPO (2025): $273.1M, investors not publicly disclosed

Key Investors in BillionToOne

Neotribe Ventures

A Silicon Valley-based venture capital firm that identifies and funds entrepreneurs developing breakthrough technologies with the potential to create transformative market movements.

Hummingbird Ventures

A global seed investment fund that focuses on investing in outlier founders at the earliest stages of their ventures, providing significant support throughout the company lifecycle.

Adams Street Partners

A private markets investment management firm founded in 1972 that provides global investment services across various stages of company growth.

What's next for BillionToOne?

With the support of major institutional backers, BillionToOne is poised to scale its diagnostic operations and expand the clinical applications of its QCT platform. The recent capital infusion provides the necessary runway to accelerate research and development, potentially broadening the scope of its screening capabilities beyond current offerings. As the company matures, the focus will likely shift toward commercial expansion and the integration of its molecular counting technology into standard clinical workflows, further solidifying its role as a transformative force in precision medicine.

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Frequently Asked Questions Regarding BillionToOne Financial Insights

What are the most recent funding rounds that BillionToOne has completed, and what were the funding rounds?
BillionToOne has recently completed 3 funding rounds: IPO on Nov 5, 2025, Series D on Jun 21, 2024, Unspecified on Dec 15, 2022.
What is the total amount of funding BillionToOne has raised to date?
BillionToOne has raised a total of $646.6M in funding to date.
How many funding rounds has BillionToOne completed?
BillionToOne has completed 3 funding rounds.
How much funding did BillionToOne raise in its most recent funding round?
BillionToOne raised $273.1M in its most recent funding round.
Which was the largest funding round in BillionToOne's history?
The largest funding round in BillionToOne's history was $273.1M.
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