How Much Did BillionToOne Raise?
Funding & Key Investors

BillionToOne, a precision diagnostics company, has secured substantial capital, with total funding reaching $646.6M and a recent strategic investment of $273.1M. This significant financial backing underscores the company's advancements in molecular diagnostics and its potential to revolutionize patient care through accurate, efficient, and accessible testing solutions.

What is BillionToOne?

BillionToOne
Business ServicesResearch & DevelopmentHealthcare Services

Headquartered in Menlo Park, California, BillionToOne is at the forefront of precision diagnostics, driven by its mission to enhance the accuracy, efficiency, and accessibility of molecular diagnostics. The company's proprietary QCT molecular counter platform is distinguished by its unique ability to count DNA molecules down to the single-count level. This groundbreaking technology underpins its flagship product, UNITY, which offers the first and only noninvasive prenatal screening capable of detecting cystic fibrosis (CF), spinal muscular atrophy (SMA), and hemoglobinopathies by analyzing cell-free fetal DNA from maternal blood. This innovative approach positions BillionToOne as a key player in the rapidly evolving landscape of diagnostic technology.

How much funding has BillionToOne raised?

BillionToOne has raised a total of $646.6M across 6 funding rounds:

2019

Series A

$15M

2021

Series B

$55M

2022

Series C

$125M

Unspecified

$48.5M

2024

Series D

$130M

2025

IPO

$273.1M

Series A (2019): $15M with participation from Libertus Capital, NeoTribe Ventures, Civilization Ventures, Pacific 8 Ventures, Hummingbird Ventures, HOF Capital, Y Combinator, and 500 Startups Istanbul

Series B (2021): $55M, investors not publicly disclosed

Series C (2022): $125M supported by Adams Street Partners, Baillie Gifford, Neotribe Ventures, and Hummingbird Ventures

Unspecified (2022): $48.5M featuring Baillie Gifford, Hummingbird Ventures, Adams Street Partners, Libertus Capital, and Neotribe Ventures

Series D (2024): $130M, investors not publicly disclosed

IPO (2025): $273.1M, investors not publicly disclosed

Key Investors in BillionToOne

Hummingbird Ventures

Hummingbird Ventures is a global seed investment fund that focuses on investing in outlier founders at the earliest stages of their ventures, providing significant support throughout the lifecycle of the companies they back.

Neotribe Ventures

Neotribe Ventures is a Silicon Valley-based venture capital firm founded in 2017 that identifies and funds entrepreneurs developing breakthrough technologies with the potential to create transformative market movements.

Adams Street Partners

Founded in 1972, Adams Street Partners provides investment management services in private markets worldwide, with its headquarters located in Chicago, Illinois.

What's next for BillionToOne?

With its latest major enterprise-level funding and a recent strategic investment, BillionToOne is poised for significant expansion and further development of its QCT platform and UNITY product line. The substantial capital infusion will likely fuel advancements in research and development, enhance manufacturing capabilities, and broaden market penetration. This strategic financing indicates a strong investor confidence in BillionToOne's technology and its potential to disrupt the diagnostics market, paving the way for broader clinical adoption and the introduction of new diagnostic tests.

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Frequently Asked Questions Regarding BillionToOne Financial Insights

What are the most recent funding rounds that BillionToOne has completed, and what were the funding rounds?
BillionToOne has recently completed 3 funding rounds: IPO on Nov 5, 2025, Series D on Jun 21, 2024, Unspecified on Dec 15, 2022.
What is the total amount of funding BillionToOne has raised to date?
BillionToOne has raised a total of $646.6M in funding to date.
How many funding rounds has BillionToOne completed?
BillionToOne has completed 3 funding rounds.
How much funding did BillionToOne raise in its most recent funding round?
BillionToOne raised $273.1M in its most recent funding round.
Which was the largest funding round in BillionToOne's history?
The largest funding round in BillionToOne's history was $273.1M.
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