Enzyme Research Laboratories

Enzyme Research Laboratories, Inc. makes the following suggestions for the storage and handling of purified proteins: Most purified proteins are stable at low temperatures and should be stored at -60C. Lyophilized proteins should be reconstituted using a good laboratory quality H2O. After reconstitution the protein should be aliquoted into a useful... (one time use) size and stored at -60C. Freeze proteins in a concentrated form and as soon as possible. When dilution is necessary, dilute immediately prior to use. When possible, dilution buffers should contain stabilizers such as albumin. Limit thawing and refreezing. Multiple thaw/freezes can reduce protein activity and cause degradation. We always suggest aliquoting the protein into a single use size. Refrigerate thawed proteins or store on ice. Proteins warmed even to room temperature may degrade quickly. One exception to # 5 is Fibrinogen. After thawing Fibrinogen, keep at room temperature. Fibrinogen will come out of solution if it is cooled Products provided in Glycerol/buffer solutions should be stored at -20C. Temperatures below -30C can cause phase transitions and need to be avoided.Read More