Dr. Wenjin Yu

Dr. Wenjin Yu Research Associate

Dr. Wenjin Yu, University of Guelph (Canada)

ISSUES AND CONCERNSAnalytical Assays to Monitor the Influence of Production Parameters on Product Consistency.
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Dr. Wenjin Yu, Department of Plant Agriculture, University of GuelphAffinity chromatography is an extensively used method for antibody purification when a pure antigen is available.However, the antibody recovery will decrease remarkably if the antigen is in a denatured form due to losing of antigen-binding capacity of the column.Here we report a very simple, inexpensive, and reliable method to purify antibodies using either denatured or native antigens.With this method we have successfully purified the polyclonal antibodies, specific to a cysteine proteinase in Vicia faba L., from crude preparation of IgY of chicken eggs.Compared with the classic purification method, the antibody recovery was tenfold higher with this method when the denatured antigen was used.

Dr. Wenjin Yu, University of Guelph (Canada)

ISSUES AND CONCERNSAnalytical Assays to Monitor the Influence of Production Parameters on Product ConsistencyInfluence of Monoclonal Antibody Manufacturing Processes on Product StabilityDesign, Implementation, Validation, and Application of Test MethodsValidation of a Chemiluminescent Hybridization Assay for Host Cell DNAPerformance Monitoring for Improved Bioprocess Understanding and Supervision
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Dr. Wenjin Yu, Department of Plant Agriculture, University of GuelphAffinity chromatography is an extensively used method for antibody purification when a pure antigen is available.However, the antibody recovery will decrease remarkably if the antigen is in a denatured form due to losing of antigen-binding capacity of the column.Here we report a very simple, inexpensive, and reliable method to purify antibodies using either denatured or native antigens.With this method we have successfully purified the polyclonal antibodies, specific to a cysteine proteinase in Vicia faba L., from crude preparation of IgY of chicken eggs.Compared with the classic purification method, the antibody recovery was tenfold higher with this method when the denatured antigen was used.