Dr. Douglas R. Smith Ph.D. This is Me

Education

 View all 22 references Web References

1. 1. DOE Microbial Genome Program Report

   www.microbialgenome.org/2000re - [Cached]

   Published on: 4/11/2006   Last Visited: 8/26/2006

   Douglas Smith
   
   Genome and Technology Development; Genome Therapeutics Corp.; 100 Beaver St.; Waltham, MA 02154-8440 781/398-2378 or /893-5007 (ext. 219), Fax: /893-9535 or /642-0310, doug.smith@genomecorp.com

2. 2. Human Genetic Variation - October 16-18, 2000, Wyndham Franklin Plaza Hotel, Philadelphia, Pennsylvania

   www.chealth.com/conference/00g - [Cached]

   Published on: 9/5/1999   Last Visited: 8/13/2000

   Dr. Douglas R. Smith, Genome Therapeutics Corporation
   ...
   Dr. Douglas R. Smith, Director, GTC Sequencing Center & Technology Development, Genome Therapeutics Corporation We have developed an assay to detect single nucleotide polymorphisms (SNPs) based on 3' end-tagging and exonuclease proofreading. The assay uses only one enzyme, and allows positive identification of the polymorphic nucleotide with a small number of procedural steps (the exact number depending on the read-out format). The efficacy of the assay has been demonstrated using fluorophore-tagged oligonucleotides from the NAT2 gene and read-out on ABI-377 instruments. The assay exhibits high sensitivity and selectivity, based on its ability to readily discriminate G : T mismatches in genomic DNA samples. The chemical characteristics of the tagged oligonucleotides allow, in principle, efficient tag-on synthesis of any sequence with a minimal number of reagents and synthetic steps. We are currently testing the assay with 3' electrophore release tags in the mass range of 200-1000 Daltons that are being developed by our collaborators at Northeastern University.

3. 3. GENOME THERAPEUTICS PUBLISHES COMPLETE GENOME SEQUENCE OF METHANOBACTERIUM THERMOAUTOTROPHICUM

   www.fkhealth.com/Releases/Geno - [Cached]

   Published on: 11/11/1997   Last Visited: 4/29/2001

   These findings will provide valuable insight into specific genes , their functions and biological pathways , said Douglas R. Smith , Ph.D. , principal investigator and Senior Research Fellow , Genome Therapeutics. A total of 1 , 855 protein encoding genes have been identified , and approximately 44 % have been assigned putative functions. There is extensive genetic divergence between Methanobacterium and Methanococcus , with less than 20 % of the open reading frames encoding sequences that are more than 50 % identical..