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This profile was automatically generated using 6 references found on the Internet. This information has not been verified. Learn more...
This profile was automatically generated using 6 references found on the Internet. This information has not been verified. Learn more...
View all 6 references Web References
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1. www.genengnews.com
www.genengnews.com/articles/ch - [Cached]Published on: 3/15/2008 Last Visited: 3/20/2008
"They are, however, ion exchangers, and could be used for capture as well," points out Peter Levison, Ph.D., technology development director at Pall's Portsmouth, U.K., facility. -
2. Data Demonstrates Unique Advantages of New Mixed-Mode Sorbents in Process Chromatography
domino.pall.com/news_articles_ - [Cached]Published on: 5/15/2006 Last Visited: 3/20/2008
Peter R, Levison, Ph.D., Technology Development Director, Pall Corporation (NYSE: PLL) is presenting data on a unique new mixed-mode chromatography method combining hydrophobic and ionic components that give process chromatographers unprecedented versatility in protein purification, while also significantly reducing costs and environmental burdens. He is presenting the results of this study as part of the scientific program at the 19th International PREP Symposia in Baltimore, Maryland today.
The new Pall BioSepra® PPA HyperCelâ„¢ and HEA HyperCelâ„¢ mixed-mode chromatography sorbents are capable of interacting with a range of proteins under physiological-type conditions (pH and ionic strength) without the need to add lyotropic salts at concentrations typical of those required for traditional HIC. The added lyotropes require disposal following the adsorption and wash stages, resulting in additional expense beyond initial reagent costs, and can also significantly impact the environment, especially with large-scale production.
Dr. Levison explains that the combined characteristics of HIC and ion exchange chromatography (IEX) in the new sorbents allow process chromatographers to adjust modalities to capture virtually any protein. He shared data that shows the superior binding capacity of the new sorbents compared to conventional HIC sorbents. For example, the mixed mode sorbents exhibited a binding capacity of ~50mg/ml for BSA in phosphate buffered saline, whereas no significant BSA binding capacity was observed for HIC chromatography under similar physiological conditions.
"HEA HyperCel and PPA HyperCel add universal protein purification tools to the Pall process chromatography toolbox," says Dr. Levison.
...
"Eliminating the need to add lyotropic salts provides a considerable economic and environmental benefit for chromatography operations," says Dr. Levison.
...
Peter R. Levison, PhD. will present the study "Physiological Hydrophobic Interaction Chromatography. A New Technique for Process Applications" on May 16 from 4:30pm-4:50pm. -
3. Data Demonstrates Unique Advantages of New Mixed-Mode Sorbents in Process Chromatography
www.pallindia.com/news_article - [Cached]Published on: 5/15/2006 Last Visited: 3/8/2008
Peter R, Levison, Ph.D., Technology Development Director, Pall Corporation (NYSE: PLL) is presenting data on a unique new mixed-mode chromatography method combining hydrophobic and ionic components that give process chromatographers unprecedented versatility in protein purification, while also significantly reducing costs and environmental burdens. He is presenting the results of this study as part of the scientific program at the 19th International PREP Symposia in Baltimore, Maryland today.
The new Pall BioSepra® PPA HyperCelâ„¢ and HEA HyperCelâ„¢ mixed-mode chromatography sorbents are capable of interacting with a range of proteins under physiological-type conditions (pH and ionic strength) without the need to add lyotropic salts at concentrations typical of those required for traditional HIC. The added lyotropes require disposal following the adsorption and wash stages, resulting in additional expense beyond initial reagent costs, and can also significantly impact the environment, especially with large-scale production.
Dr. Levison explains that the combined characteristics of HIC and ion exchange chromatography (IEX) in the new sorbents allow process chromatographers to adjust modalities to capture virtually any protein. He shared data that shows the superior binding capacity of the new sorbents compared to conventional HIC sorbents. For example, the mixed mode sorbents exhibited a binding capacity of ~50mg/ml for BSA in phosphate buffered saline, whereas no significant BSA binding capacity was observed for HIC chromatography under similar physiological conditions.
"HEA HyperCel and PPA HyperCel add universal protein purification tools to the Pall process chromatography toolbox," says Dr. Levison.
...
"Eliminating the need to add lyotropic salts provides a considerable economic and environmental benefit for chromatography operations," says Dr. Levison.
...
Peter R. Levison, PhD. will present the study "Physiological Hydrophobic Interaction Chromatography. A New Technique for Process Applications" on May 16 from 4:30pm-4:50pm.
