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2006-09-04T00:00:00.000Z

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Porter D. Porter

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Background Information

Employment History

Member, Bacteriology Department

Bacteriology Department of Genetics Biotechnology Center

Biochemistry Genome Expression Center

Pasadena , CA

Web References (3 Total References)


1 Nuwaysir EF Emile F. Nuwaysir ...

www.genefrontier.com [cached]

1 Nuwaysir EF Emile F. Nuwaysir 1 2 Huang W Wei Huang 2 3 Albert TJ Thomas J. Albert 1 4 Singh J Jaz Singh 1 5 Nuwaysir K Kate Nuwaysir 1 6 Pitas A Alan Pitas 1 7 Richmond T Todd Richmond 1 8 Gorski T Tom Gorski 1 9 Berg JP James P. Berg 1 10 Ballin J Jeff Ballin 2 11 McCormick M Mark McCormick 1 12 Norton J Jason Norton 1 13 Pollock T Tim Pollock 1 14 Sumwalt T Terry Sumwalt 1 15 Butcher L Lawrence Butcher 1 16 Porter D DeAnn Porter 1 17 Molla M Michael Molla 3 18 Hall C Christine Hall 4 19 Blattner F Fred Blattner 5 20 Sussman MR Michael R. Sussman 6 21 Wallace RL Rodney L. Wallace 1 22 Cerrina F Franco Cerrina 2 23 Green RD Roland D. Green 1 NimbleGen Systems, Inc., Madison, WI 53711 Center for NanoTechnology (Department of Electrical Engineering and Computer Engineering Computer Sciences Department, Department of Bacteriology Department of Genetics Biotechnology Center, University of Wisconsin, Madison, WI 53706

...
Solution studies strongly corroborated the sequence preferences identified by the array analysis.1 Warren CL Christopher L. Warren 1,2 2 Kratochvil NC Natasha C. S. Kratochvil 1 3 Hauschild KE Karl E. Hauschil 1 4 Foister S Shane Foister 3 5 Brezinski ML Mary L. Brezinski 1 6 Dervan PB Peter B. Dervan 3 7 Phillips GN Jr George N. Phillips, Jr. 1,2 8 Ansari AZ Aseem Z. Ansari 1,2 Department of Biochemistry and Genome Center, University of Wisconsin, Madison, WI Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA
...
Libraries of insertion mutants generated by a unique Tn5 mutagenesis system were grown competitively in defined media.Biotin-labeled runoff RNA transcripts were generated in vitro from transposon insertions in each population of mutants.These transcripts were then hybridized to custom-designed oligonucleotide microarrays to detect the presence of each mutant in the population.By using this approach, the signal associated with 25 auxotrophic insertions in a 50-mutant pool was not detectable following nine generations of growth in glucose M9 minimal medium.It was found that individual insertion sites could be mapped to within 50 bp of their genomic locations, and 340 dispensable regions in the E. coli chromosome were identified.Tn5 insertions were detected in 15 genes for which no previous insertions have been reported.Other applications of this method are discussed.1 Winterberg KM Kelly M. Winterberg 1 2 Luecke J John Luecke 2 3 Bruegl AS Amanda S. Brueg 3 4 Reznikoff WS William S. Reznikoff 1,4 Department of Biochemistry Genome Expression Center, University of Wisconsin-Madison, Madison, Wisconsin University of Washington-Seattle, Seattle, Washington Corresponding author.Mailing address: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Room 319, Madison, WI 53706-1544.Phone: (608) 262-3608.Fax: (608) 265-2603.E-mail: reznikoff@biochem.wisc.edu
On MAS Technology Recent Highlights on Photolytic Oligonucleotide Array in situ SynthesisNucleosides Nucleotides Nucleic Acids 2005;24(5-7):891-6 2005-01-01
2005-01-01 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16248056&query_hl=65&itool=pubmed_docsum Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc.2005-01-01 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16248056&query_hl=65&itool=pubmed_docsum Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost.In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used.If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign.The arrays synthesized show high quality and reproducibility in our standard hybridization based assay.1 Stengele KP Stengele KP 1 2 Buhler J B,hler J 1 3 Buhler S B,hler S 1 4 Kvassiouk E Kvassiouk E 1 5 Green R Green R 1 6 Prykota T Prykota T 1 7 Pfleiderer W and Pfleiderer W 1 NimbleGen Systems GbmH, Waldkreiburg, Germany


Comprehensive DNA methylation profiling in a human cancer genome identifies novel epigenetic targets

www.nimblegen.com [cached]

1 Nuwaysir EF Emile F. Nuwaysir 1 2 Huang W Wei Huang 2 3 Albert TJ Thomas J. Albert 1 4 Singh J Jaz Singh 1 5 Nuwaysir K Kate Nuwaysir 1 6 Pitas A Alan Pitas 1 7 Richmond T Todd Richmond 1 8 Gorski T Tom Gorski 1 9 Berg JP James P. Berg 1 10 Ballin J Jeff Ballin 2 11 McCormick M Mark McCormick 1 12 Norton J Jason Norton 1 13 Pollock T Tim Pollock 1 14 Sumwalt T Terry Sumwalt 1 15 Butcher L Lawrence Butcher 1 16 Porter D DeAnn Porter 1 17 Molla M Michael Molla 3 18 Hall C Christine Hall 4 19 Blattner F Fred Blattner 5 20 Sussman MR Michael R. Sussman 6 21 Wallace RL Rodney L. Wallace 1 22 Cerrina F Franco Cerrina 2 23 Green RD Roland D. Green 1 NimbleGen Systems, Inc., Madison, WI 53711 Center for NanoTechnology (Department of Electrical Engineering and Computer Engineering Computer Sciences Department, Department of Bacteriology Department of Genetics Biotechnology Center, University of Wisconsin, Madison, WI 53706

...
Solution studies strongly corroborated the sequence preferences identified by the array analysis. 1 Warren CL Christopher L. Warren 1,2 2 Kratochvil NC Natasha C. S. Kratochvil 1 3 Hauschild KE Karl E. Hauschil 1 4 Foister S Shane Foister 3 5 Brezinski ML Mary L. Brezinski 1 6 Dervan PB Peter B. Dervan 3 7 Phillips GN Jr George N. Phillips, Jr. 1,2 8 Ansari AZ Aseem Z. Ansari 1,2 Department of Biochemistry and Genome Center, University of Wisconsin, Madison, WI Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA
...
Libraries of insertion mutants generated by a unique Tn5 mutagenesis system were grown competitively in defined media. Biotin-labeled runoff RNA transcripts were generated in vitro from transposon insertions in each population of mutants. These transcripts were then hybridized to custom-designed oligonucleotide microarrays to detect the presence of each mutant in the population. By using this approach, the signal associated with 25 auxotrophic insertions in a 50-mutant pool was not detectable following nine generations of growth in glucose M9 minimal medium. It was found that individual insertion sites could be mapped to within 50 bp of their genomic locations, and 340 dispensable regions in the E. coli chromosome were identified. Tn5 insertions were detected in 15 genes for which no previous insertions have been reported. Other applications of this method are discussed. 1 Winterberg KM Kelly M. Winterberg 1 2 Luecke J John Luecke 2 3 Bruegl AS Amanda S. Brueg 3 4 Reznikoff WS William S. Reznikoff 1,4 Department of Biochemistry Genome Expression Center, University of Wisconsin-Madison, Madison, Wisconsin University of Washington-Seattle, Seattle, Washington Corresponding author. Mailing address: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Room 319, Madison, WI 53706-1544. Phone: (608) 262-3608. Fax: (608) 265-2603. E-mail: reznikoff@biochem.wisc.edu
On MAS Technology Recent Highlights on Photolytic Oligonucleotide Array in situ Synthesis Nucleosides Nucleotides Nucleic Acids 2005;24(5-7):891-6 2005-01-01
2005-01-01 http://dx.doi.org/10.1081/NCN-200059241 Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc. 2005-01-01 http://dx.doi.org/10.1081/NCN-200059241 Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost. In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used. If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign. The arrays synthesized show high quality and reproducibility in our standard hybridization based assay. 1 Stengele KP Stengele KP 1 2 Buhler J B,hler J 1 3 Buhler S B,hler S 1 4 Kvassiouk E Kvassiouk E 1 5 Green R Green R 1 6 Prykota T Prykota T 1 7 Pfleiderer W and Pfleiderer W 1 NimbleGen Systems GbmH, Waldkreiburg, Germany


1 Nuwaysir EF Emile F. Nuwaysir ...

www.genefrontier.com [cached]

1 Nuwaysir EF Emile F. Nuwaysir 1 2 Huang W Wei Huang 2 3 Albert TJ Thomas J. Albert 1 4 Singh J Jaz Singh 1 5 Nuwaysir K Kate Nuwaysir 1 6 Pitas A Alan Pitas 1 7 Richmond T Todd Richmond 1 8 Gorski T Tom Gorski 1 9 Berg JP James P. Berg 1 10 Ballin J Jeff Ballin 2 11 McCormick M Mark McCormick 1 12 Norton J Jason Norton 1 13 Pollock T Tim Pollock 1 14 Sumwalt T Terry Sumwalt 1 15 Butcher L Lawrence Butcher 1 16 Porter D DeAnn Porter 1 17 Molla M Michael Molla 3 18 Hall C Christine Hall 4 19 Blattner F Fred Blattner 5 20 Sussman MR Michael R. Sussman 6 21 Wallace RL Rodney L. Wallace 1 22 Cerrina F Franco Cerrina 2 23 Green RD Roland D. Green 1 NimbleGen Systems, Inc., Madison, WI 53711 Center for NanoTechnology (Department of Electrical Engineering and Computer Engineering Computer Sciences Department, Department of Bacteriology Department of Genetics Biotechnology Center, University of Wisconsin, Madison, WI 53706

...
Solution studies strongly corroborated the sequence preferences identified by the array analysis.1 Warren CL Christopher L. Warren 1,2 2 Kratochvil NC Natasha C. S. Kratochvil 1 3 Hauschild KE Karl E. Hauschil 1 4 Foister S Shane Foister 3 5 Brezinski ML Mary L. Brezinski 1 6 Dervan PB Peter B. Dervan 3 7 Phillips GN Jr George N. Phillips, Jr. 1,2 8 Ansari AZ Aseem Z. Ansari 1,2 Department of Biochemistry and Genome Center, University of Wisconsin, Madison, WI Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA
...
Libraries of insertion mutants generated by a unique Tn5 mutagenesis system were grown competitively in defined media.Biotin-labeled runoff RNA transcripts were generated in vitro from transposon insertions in each population of mutants.These transcripts were then hybridized to custom-designed oligonucleotide microarrays to detect the presence of each mutant in the population.By using this approach, the signal associated with 25 auxotrophic insertions in a 50-mutant pool was not detectable following nine generations of growth in glucose M9 minimal medium.It was found that individual insertion sites could be mapped to within 50 bp of their genomic locations, and 340 dispensable regions in the E. coli chromosome were identified.Tn5 insertions were detected in 15 genes for which no previous insertions have been reported.Other applications of this method are discussed.1 Winterberg KM Kelly M. Winterberg 1 2 Luecke J John Luecke 2 3 Bruegl AS Amanda S. Brueg 3 4 Reznikoff WS William S. Reznikoff 1,4 Department of Biochemistry Genome Expression Center, University of Wisconsin-Madison, Madison, Wisconsin University of Washington-Seattle, Seattle, Washington Corresponding author.Mailing address: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Room 319, Madison, WI 53706-1544.Phone: (608) 262-3608.Fax: (608) 265-2603.E-mail: reznikoff@biochem.wisc.edu
On MAS Technology Recent Highlights on Photolytic Oligonucleotide Array in situ SynthesisNucleosides Nucleotides Nucleic Acids 2005;24(5-7):891-6 2005-01-01
2005-01-01 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16248056&query_hl=65&itool=pubmed_docsum Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc.2005-01-01 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16248056&query_hl=65&itool=pubmed_docsum Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block.
...
Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost.In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used.If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign.The arrays synthesized show high quality and reproducibility in our standard hybridization based assay.1 Stengele KP Stengele KP 1 2 Buhler J B,hler J 1 3 Buhler S B,hler S 1 4 Kvassiouk E Kvassiouk E 1 5 Green R Green R 1 6 Prykota T Prykota T 1 7 Pfleiderer W and Pfleiderer W 1 NimbleGen Systems GbmH, Waldkreiburg, Germany

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