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This profile was last updated on 5/27/15  and contains information from public web pages.

Research Scientist, Adjunct Profe...

Phone: (519) ***-**** ext. *****  
Local Address:  Ontario , Canada
University of Guelph
50 Stone Road East
Guelph , Ontario N1G 2W1

Company Description: The University of Guelph is one of the most research-intensive universities in Canada and is internationally renowned for its broad spectrum of research. The...   more

Employment History

15 Total References
Web References
Directory | Plant Agriculture, 27 May 2015 [cached]
Laima Kott Research Scientist (retired), Adjunct Professor (519) 824-4120 x53572
Laima Kott | Department of Plant Agriculture | University of Guelph, 29 Oct 2012 [cached]
Laima Kott Research Scientist (retired), Adjunct Professor
Laima Kott
Laima Kott, Research Scientist (retired), Adjunct Professor
Kott, L.S., R.S. Fletcher and K. Perry. (2004).
Kott, L.S. and L. Dosdall. (2004). Introgression of root maggot resistance (Delia spp.) derived from Sinapis alba L. into Brassica napus L. Brassica 6 (3&4): 55-62.
Kott, L.S., I. Kyrychenko and R. Fletcher. (2002).
Centralia Swine Research Update - Proceedings 2007, 31 Jan 2007 [cached]
Laima Kott, Dept. of Plant Agriculture, University of Guelph
All photos courtesy of Dr. Laima ..., 17 Sept 2009 [cached]
All photos courtesy of Dr. Laima Kott, University of Guelph.
It was originally developed for canola in the mid-1980s by Dr. Laima Kott, researcher and adjunct professor in the Department of Plant Agriculture at the University of Guelph. Although similar culture systems have since been established for barley and rice, corn has been notoriously difficult to manipulate in such a system. However, Kott and laboratory technician Ecaterina Simion, who have a combined total of about 50 years' experience with microspore culture, are getting close to completing a functional microspore culture system for corn after almost three years of work.
"We extract the pollen grains out of the anthers so they're loose, and then we get rid of the debris and put these pollen grains into liquid medium in a Petri dish, where they can start growing," says Kott. This can yield hundreds to millions of tiny pollen grains per dish.
Before the pollen grains start to grow, they are exposed to ultraviolet (UV) light for a specific amount of time. This creates small mutations, called point mutations, in which only a small number of the DNA building blocks are altered. Many of these mutations will be lethal, but some will not. "Because we have a million or so pollen grains per plate, there's no problem with how much tissue we have to work with; it's totally abundant," explains Kott.
"So what we've done is basically killed everything that didn't have the right mutation and kept the ones that have the mutation that we prefer," says Kott.
Each selection agent alone kills 99 percent or more of the embryos, so they are not combined in the screening tests, says Kott.
"Fusarium is just one trait; there are other things we can screen for," says Kott.
For this reason, Hyland Seeds, Dr. Laima Kott and technician Ecaterina Simion at the Department of Plant Agriculture, University of Guelph, are also exploring microspore culture in wheat.
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