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Wrong Anne Ruffing?

Anne M. Ruffing

Senior Member of the Technical Staff

Sandia National Laboratories

HQ Phone:  (505) 293-0500

Direct Phone: (505) ***-****direct phone

Email: a***@***.gov

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I agree to the Terms of Service and Privacy Policy. I understand that I will receive a subscription to ZoomInfo Community Edition at no charge in exchange for downloading and installing the ZoomInfo Contact Contributor utility which, among other features, involves sharing my business contacts as well as headers and signature blocks from emails that I receive.

Sandia National Laboratories

3707 Juan Tabo Blvd NE

Albuquerque, New Mexico,87111

United States

Company Description

Sandia National Laboratories is a multi-program laboratory operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Company, for the U.S. Department of Energy's National Nuclear Security Administration. With main facilities in Albuquerque, ...more

Web References(23 Total References)


Summit Agenda - Algae Biomass Organization

www.algaebiomasssummit.org [cached]

Moderator: Anne Ruffing, Sandia National Labs


Frontiers

www.frontiersin.org [cached]

Anne M Ruffing
Anne M Ruffing Sandia National Laboratories


www.algaebiomasssummit.org

Anne Ruffing, Sandia National Laboratories
Building a Synthetic Biology Toolbox for Synechococcus sp. PCC 7002


www.rdmag.com

Sandia National Laboratories Truman Fellow Anne Ruffing has engineered two strains of cyanobacteria to produce free fatty acids, a precursor to liquid fuels, but she has also found that the process cuts the bacteria's production potential.


www.robaid.com

Anne Ruffing, Truman Fellow at Sandia's Bioenergy and Defense Technologies Department, studied the direct conversion of carbon dioxide into biofuels by photosynthetic organisms such as algae.
Instead focusing on eukaryotic algae, the natural "oil"-producing photosynthetic microorganisms, she used performed her research on cyanobacteria (blue-green algae) since they are easier to genetically manipulate, and because cyanobacteria can be engineered to create a variety of target fuels. "You can't really hope to continue to engineer it to produce more of the fatty acids until you address these unforeseen effects", said Ruffing. "As much as you want to do the applied side of things, creating the strain, you can't get away from the fundamental biology that's necessary in order to do that." The strains she engineered for FFA production show reduced photosynthetic yields, degradation of chlorophyll-a and changes in light-harvesting pigments. Ruffing saw some cell death and lower growth rates overall, and suspects that the toxicity of unsaturated FFA and changes in membrane composition are responsible. She chose Synechococcus elongatus PCC 7942 and Synechococcus sp. PCC 7002 as so-called model organisms since they have been studied for several decades and have established tools for gene manipulation. Aside model organisms, she's performing biofuel toxicity screening on Synechocystis sp. PCC 6803. Ruffing is creating mutants by knocking out certain genes or introducing or overexpressing genes to see how that affects the cell and fatty acid production.


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