Neuroproteomics

Revenues:

Under $20 Million

Description

The Neuroproteomics laboratory (NPL) is designed to provide an entry point for proteomics projects. The laboratory consists of two major workflows: 2D gel Electrophoresis (2DE) and Multidimensional Liquid Chromatography (MDLC).

The NPL is one of the major components of the Center for Translational Neurosciences (CTN), an initiative of the Department of Neurobiology and the Division of Neurology. The NPL is open to all Duke University investigators, with priority access to Neurobiology and Neurology members. The NPL is responsible for providing Duke researchers current and novel technologies for protein analysis and for basic research in Neuroproetomics. Laboratory personnel provide detailed assistance and consultation for sample preparation including specific protocols and methodologies with particular emphasis on protein isolation from nervous tissues. 2DE services provided include running samples on 2D gels, spot visualization and quantification using fluorescent protein staining and imaging, and spot picking for subsequent mass spectrometric analysis in the Mass Spectrometry Laboratory directed by Dr. Tim Haystead.

Subcellular fractionation and MDLC will be available on a project-oriented basis in consultation with NPL personnel. Detailed protocols, consultation, and direct assistance will be available for isolating proteins from nervous tissues. The NPL features the Differential in Gel Expression (DIGE) analysis method, which is a powerful approach for the determination of protein expression in any tissue, compared to a control sample. The next is a description of the DIGE system and the work-flow for 2DE in the Neuroproteomics Laboratory.

News Archive

Research on Alzheimer disease detailed by B. Kovacech and co-authors. Pain & Central Nervous System Week; 10/5/2009 ...therapeutic targets for AD. Functional neuroproteomics was employed to generate the first...neurofibrillary degeneration. Structural neuroproteomics allowed us to determine partial 3D...a resolution of 1.6. Signaling neuroproteomics data lead to the identification and... ...more